This book aims to describe some of the important recent developments in plant genotyping. It is based upon a second workshop held recently to review progress in this area. Recent developments focus on high- throughput methods and generally target single nucleotide polymorphism (SNP) discovery and analysis. The topics covered include: SNP discovery in plants; SNPs and their use in maize; rare SNP discovery with endonucleases; sequence polymorphisms in the flanking regions of microsatellite markers; SNP discovery by ecotilling using capillary electrophoresis; genotyping by allele-specific PCR; the MassARRAY system for plant genomics; mutation screening; nanotechnology (the future of cost-effective plant genotyping); functionally associated molecular genetic markers for temperate pasture plant improvement; genotyping for rice eating qualities; towards universal loci for plant genotyping; DNA banks as a resource for SNP genotyping; DNA extraction from plant tissue; future prospects for plant genotyping.